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And could improve screening uptake, say experts

Research: Accuracy of urinary human papillomavirus detection for viral surveillance and cervical cancer screening: systematic review and meta-analysis

A simple urine test for human papillomavirus (HPV) could offer a more acceptable, non-invasive alternative to the conventional cervical test and improve screening uptake, finds a study published on thebmj.com today.

Human papillomavirus (HPV) is one of the most common sexually transmitted infections. Up to 80% of sexually active women are infected at some point in their lives and infection with specific “high risk” strains of HPV has an established link to cervical cancer.

Current screening by cervical cytology (smear test) is invasive and time-consuming – and in recent years, cervical screening in the UK has fallen below 80%, particularly amongst women aged 25-30. Several studies have suggested that detecting HPV in urine may be a feasible alternative to cervical sampling, but the accuracy of such a test is still uncertain.

So a team of researchers based in London and Spain analysed the results of 14 studies involving 1,443 sexually active women to determine the accuracy of testing for HPV on urine samples compared with cervical samples obtained by a doctor.

The quality of the studies was generally high.

HPV virus
Compared with cervical samples, urine HPV testing had an overall sensitivity of 87% (the proportion of positives correctly identified) and a specificity of 94% (the proportion of negatives correctly identified). Urine testing for “high risk” HPV types 16 and 18 (those that cause the majority of cervical cancer cases) had an overall sensitivity of 73% and a specificity of 98% compared with cervical samples.

Accuracy increased when “first-void” urine samples were collected (the first urine of the day) compared with random or mid-stream samples, probably because first void urine samples contain higher levels of DNA.

“The detection of HPV in urine is non-invasive, easily accessible, and acceptable to women, and a test with these qualities could considerably increase uptake,” say the authors.

They stress that their results should be interpreted with caution “due to variation between individual studies,” but say that when HPV testing is considered for cervical cancer screening, “urine-based testing should be considered as an accurate and acceptable alternative that could increase screening coverage.”

They propose that testing urine for HPV “could accurately replace cervical testing for HPV in this context” and call for further studies and a “standardised method of urine HPV detection” to be developed.

In an accompanying editorial, researchers at the University of Manchester say urine testing for HPV is a promising screening option that deserves further evaluation.

In well resourced health systems, they suggest self sampling “could be used for women who are reluctant to attend for regular cervical screening.” While in lower income countries that lack infrastructure, “self sampling might even be beneficial and cost effective for all women who are eligible for screening.”

More research is now required to identify the true clinical performance and acceptability of urine testing for HPV in both settings, they conclude.

Research: Dr Neha Pathak, Women’s Health Research Unit, Centre for Primary Care and Public Health, Blizard Institute, Barts and The London School of Medicine and dentistry, Queen Mary University of London, UK

Editorial: Henry Kitchener, Professor and Chair of Gynaecological Oncology, Institute of Cancer Sciences, University of Manchester, St Mary’s Hospital, Manchester, UK

Full study: http://www.bmj.com/cgi/doi/10.1136/bmj.g5264

Source: Accuracy of urinary human papillomavirus testing for presence of cervical HPV: systematic review and meta-analysis  Neha Pathak academic foundation year 2 doctor¹, Julie Dodds senior clinical trials manager¹, Javier Zamora senior lecturer in perinatal statistics¹², Khalid Khan professor of women’s health and clinical epidemiology ¹

¹Women’s Health Research Unit, Centre for Primary Care and Public Health, Blizard Institute, Barts and The London School of Medicine and dentistry, London E1 2AB, UK ;

²Clinical Biostatistics Unit, Hospital Ramon y Cajal (IRYCIS) and CIBER Epidemiologia y Salud Publica, Madrid, Spain